Cloned (Comment) | Organism |
---|---|
subcloning in the pGEM-T vector, cloning in the pBirAR vector for overexpression or gene silencing, transformation of Nicotiana tabacum leaf discs mediated by Agrobacterium tumefaciens strain GV2260 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Nicotiana tabacum | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
calculated | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + geranylgeranyl diphosphate | Nicotiana tabacum | - |
geranylgeranylchlorophyllide a + diphosphate | - |
? | |
chlorophyllide a + phytyl diphosphate | Nicotiana tabacum | - |
chlorophyll a + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | C3W4Q1 | Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants | - |
Nicotiana tabacum | C3W4Q2 | Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
leaf segments are laid out on buffer-moistened filter paper containing Zn chlorophyllide a for 8 h in the dark, formed product is analyzed by HPLC, overexpression leads to 120-140% chlorophyll synthase activity, expression of the antisense RNA reduces activity to less than 20% and 40% | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + geranylgeranyl diphosphate | - |
Nicotiana tabacum | geranylgeranylchlorophyllide a + diphosphate | - |
? | |
chlorophyllide a + phytyl diphosphate | - |
Nicotiana tabacum | chlorophyll a + diphosphate | - |
? | |
Zn chlorophyllide a + geranylgeranyl diphosphate | - |
Nicotiana tabacum | Zn chlorophyll a + diphosphate | - |
? | |
Zn chlorophyllide a + phytyl diphosphate | - |
Nicotiana tabacum | Zn chlorophyll a + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chlorophyll synthase | - |
Nicotiana tabacum |
NtCHLG | - |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
malfunction | reduced enzyme activity does not result in accumulation of chlorophyllide, it causes reduced 5-aminolevulinic acid formation and Mg chelatase and ferrochelatase activity, growth is retarded, leaves are pale green (20% wild type chlorophyll content), overexpression is correlated with enhanced 5-aminolevulinic acid synthesizing capacity and more chelatase activity, the results point to a feedback control of the biosynthesis pathway, the phenotype is not distinguishable from the wild type | Nicotiana tabacum |
metabolism | enzyme catalyzes the last step in the chlorophyll biosynthetic pathway | Nicotiana tabacum |